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KMID : 1234420100380040428
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 4 p.428 ~ p.433
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Characterization of Agarose Produced by Yeast Cell Surface Displayed-Arylsulfatase
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Cho Eun-Soo
Kim Jeong-Hwan
Kim Yeon-Hee
Nam Soo-Wan
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Abstract
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Enzymatic hydrolysis of sulfate groups in agaropectin or agar simplifies the production process of high-quality or low sulfate-content agarose. This study was investigated that cell surface displayed arylsulfatase can be applied to desulfatation of agar for production of agarose. Sulfate content of agarose prepared by treatment of yeast surface-displayed arylsulfatase was decreased in a enzyme dosedependent manner. Especially, 35 unit/mL of yeast surface arylsulfatase attenuated sulfate content of agarose up to 0.2%. In the 0.6% agar(Junsei), 35 unit/mL enzyme treated at 40oC for 3 h showed the lowest content of sulfate. Therefore, this result was determined to be the optimal condition to desulfatation of agar for production of agarose. In addition, the gel strength of yeast surface arylsulfatase treated agar and commercial agarose were compared. Agarose prepared by treatment of yeast surface arylsulfatase showed 559.8±0.12 of gel strength, and it is a similar compared to the commercial agarose.
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KEYWORD
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Yeast cell-surface, arylsulfatase, Saccharomyces cerevisiae, desulfatation, agar, agarose
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